Tool
Reconstitution Calculator
Enter the strength printed on the vial and the volume of solvent added. The calculator gives the resulting concentration in mg/mL and mcg/mL, and the solvent volume needed to reach a target concentration. Everything is calculated in your browser; nothing is sent or stored.
How the numbers are worked out
- Concentration = vial strength ÷ solvent volume. A 10 mg vial in 2 mL gives 5 mg/mL.
- Solvent for a target concentration = vial strength ÷ target concentration. A 10 mg vial at 2 mg/mL needs 5 mL.
This tool performs arithmetic only, for laboratory preparation of research materials. NobelPepLab supplies research chemicals for in vitro and laboratory use, does not provide dosing guidance, and does not supply products for human or veterinary use. Adding solvent to lyophilised material changes how long it stays stable — see handling and storage for working life after reconstitution.
Common questions
How much bacteriostatic water do I add to a vial?
There is no single correct volume. What you add sets the concentration, and that is the only thing it fixes: 2 mL into a 10 mg vial gives 5 mg per mL, while 1 mL into the same vial gives 10 mg per mL. The usual approach is to work backwards from the concentration your method calls for. Enter the vial strength and a target concentration above and the calculator gives the solvent volume; enter a solvent volume instead and it gives the concentration.
Does bacteriostatic water contain alcohol?
Yes. Bacteriostatic water contains roughly 0.9 percent benzyl alcohol as a preservative. That is what suppresses microbial growth and allows a vial to be drawn from more than once. Sterile water has no preservative, which is the whole difference between the two.
How long does a reconstituted vial last?
Twenty-eight days is the figure conventionally applied to bacteriostatic water once the seal is broken, kept cold and out of the light. A solution made up with plain sterile water gets no such window, because there is nothing in it holding microbial growth back. The preservative is buying time, not sterility.
How long do lyophilised peptides last at room temperature?
Far longer than the same peptide in solution. The freeze-dried state is what protects the material, which is why sealed vials travel at ambient temperature as a matter of course and a few days in transit is not a problem. It is the reconstituted solution that is fragile, not the powder. Our handling and storage reference covers what a freeze-thaw cycle actually costs.
Does this work as a tesamorelin or BPC-157 calculator?
Yes, and the arithmetic does not change from one compound to another. A 10 mg tesamorelin vial and a 10 mg BPC-157 vial reconstituted with the same volume of solvent reach the same concentration, because concentration is set by vial strength and solvent volume alone. What differs between compounds is the strength they are supplied at, so read the figure on your own vial rather than assuming a standard. The same holds for TB-500, GHK-Cu, retatrutide, semaglutide and tirzepatide.
How do I calculate a blend such as BPC-157 and TB-500?
Establish what the stated strength refers to before you calculate anything. A BPC-157 and TB-500 blend labelled 10 mg may mean 10 mg of each component or 10 mg combined across both, and those two readings give very different concentrations from the same vial. The certificate of analysis and the product label are the authority on which it is — not convention, and not what a similar product from elsewhere happened to mean. Once that is settled, enter the figure that applies and the calculator treats it exactly as it would a single-compound vial.